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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
02/01/2001 |
Data da última atualização: |
07/06/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GAZIOLA, S. A.; ALESSI, E. S.; GUIMARAES, P. E. O.; DAMERVAL, C.; AZEVEDO, R. A. |
Afiliação: |
PAULO EVARISTO DE O GUIMARAES, CNPMS. |
Título: |
Quality protein maize: a biochemical study of enzymes involved in lysine metabolism. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Washington, v. 47, n. 3, p. 1268-1275, 1999. |
Idioma: |
Inglês |
Conteúdo: |
Quality protein maize (QPM) varieties have been produced by the introduction of opaque-2 modifier genes. Two QPM varieties, BR451, and BR473, a wild type and an opaque-2 variety, have beeen used to study key enzymes controlling lysine metabolism in the endosperm during development. Aspartate kinase and homoserine dehydrogenase enzymes. which are involved in lysine and threonine biosynthesis, respectively, exhibited identical activity patterns during endosperm development, with a maximum specific activity at 16 days after pollination. The QPM varieties exhibited higher levels of aspartate kinase activity in the endosperm, suggesting an increased rate of lysine biosynthesis when compared to the opaque-2 and wild-type genotypes. Similar results were observed for the lysine ketoglutarate reductase and saccharopine dehydrogenase enzymes, which form a single bifunctional polypetide involved in endosperm lysine degradation. Both enzyme activity were strongly reduced in the opaque-2 maize variety when compared to the wild-type maize, whereas the QPM varieties exhibited even lower levels of lysine ketoglutarate reductase-saccharopine dehydrogenase activities when compared to the opaque-2 variety. The developmental pattern of ensyme activity showed a different profile when compared to the enzymes involved in lysine biosynthesis, with activity being detected only 12-16 days after pollination (DAP) and maximum activities ~24 DAP. These results also suggest that the modifier genes have intensified the effect of the opaque-2 mutation on lysine ketoglutarate reductase-saccharopine dehydrogenase. These alterations lead to an increase in soluble lysine in the endosperm of the QPM varieties when compared to the opaque-2 and wild type. MenosQuality protein maize (QPM) varieties have been produced by the introduction of opaque-2 modifier genes. Two QPM varieties, BR451, and BR473, a wild type and an opaque-2 variety, have beeen used to study key enzymes controlling lysine metabolism in the endosperm during development. Aspartate kinase and homoserine dehydrogenase enzymes. which are involved in lysine and threonine biosynthesis, respectively, exhibited identical activity patterns during endosperm development, with a maximum specific activity at 16 days after pollination. The QPM varieties exhibited higher levels of aspartate kinase activity in the endosperm, suggesting an increased rate of lysine biosynthesis when compared to the opaque-2 and wild-type genotypes. Similar results were observed for the lysine ketoglutarate reductase and saccharopine dehydrogenase enzymes, which form a single bifunctional polypetide involved in endosperm lysine degradation. Both enzyme activity were strongly reduced in the opaque-2 maize variety when compared to the wild-type maize, whereas the QPM varieties exhibited even lower levels of lysine ketoglutarate reductase-saccharopine dehydrogenase activities when compared to the opaque-2 variety. The developmental pattern of ensyme activity showed a different profile when compared to the enzymes involved in lysine biosynthesis, with activity being detected only 12-16 days after pollination (DAP) and maximum activities ~24 DAP. These results also suggest that the modifier genes have i... Mostrar Tudo |
Palavras-Chave: |
Opaco-2; Opaque-2; Protein; QPM; Quality. |
Thesagro: |
Enzima; Lisina; Milho; Proteína; Qualidade; Zea Mays. |
Thesaurus Nal: |
enzymes; lysine. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02601naa a2200325 a 4500 001 1485183 005 2018-06-07 008 1999 bl uuuu u00u1 u #d 100 1 $aGAZIOLA, S. A. 245 $aQuality protein maize$ba biochemical study of enzymes involved in lysine metabolism.$h[electronic resource] 260 $c1999 520 $aQuality protein maize (QPM) varieties have been produced by the introduction of opaque-2 modifier genes. Two QPM varieties, BR451, and BR473, a wild type and an opaque-2 variety, have beeen used to study key enzymes controlling lysine metabolism in the endosperm during development. Aspartate kinase and homoserine dehydrogenase enzymes. which are involved in lysine and threonine biosynthesis, respectively, exhibited identical activity patterns during endosperm development, with a maximum specific activity at 16 days after pollination. The QPM varieties exhibited higher levels of aspartate kinase activity in the endosperm, suggesting an increased rate of lysine biosynthesis when compared to the opaque-2 and wild-type genotypes. Similar results were observed for the lysine ketoglutarate reductase and saccharopine dehydrogenase enzymes, which form a single bifunctional polypetide involved in endosperm lysine degradation. Both enzyme activity were strongly reduced in the opaque-2 maize variety when compared to the wild-type maize, whereas the QPM varieties exhibited even lower levels of lysine ketoglutarate reductase-saccharopine dehydrogenase activities when compared to the opaque-2 variety. The developmental pattern of ensyme activity showed a different profile when compared to the enzymes involved in lysine biosynthesis, with activity being detected only 12-16 days after pollination (DAP) and maximum activities ~24 DAP. These results also suggest that the modifier genes have intensified the effect of the opaque-2 mutation on lysine ketoglutarate reductase-saccharopine dehydrogenase. These alterations lead to an increase in soluble lysine in the endosperm of the QPM varieties when compared to the opaque-2 and wild type. 650 $aenzymes 650 $alysine 650 $aEnzima 650 $aLisina 650 $aMilho 650 $aProteína 650 $aQualidade 650 $aZea Mays 653 $aOpaco-2 653 $aOpaque-2 653 $aProtein 653 $aQPM 653 $aQuality 700 1 $aALESSI, E. S. 700 1 $aGUIMARAES, P. E. O. 700 1 $aDAMERVAL, C. 700 1 $aAZEVEDO, R. A. 773 $tJournal of Agricultural and Food Chemistry, Washington$gv. 47, n. 3, p. 1268-1275, 1999.
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Embrapa Milho e Sorgo (CNPMS) |
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Registros recuperados : 43 | |
2. | | GAZIOLA, S. A.; ALESSI, E. S.; GUIMARAES, P. E. O.; DAMERVAL, C.; AZEVEDO, R. A. Quality protein maize: a biochemical study of enzymes involved in lysine metabolism. Journal of Agricultural and Food Chemistry, Washington, v. 47, n. 3, p. 1268-1275, 1999.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Milho e Sorgo. |
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6. | | MEDICI, L. O.; AZEVEDO, R. A.; CANELLAS, L. P.; MACHADO, A. T.; PIMENTEL, C. Stomatal conductance of maize under water and nitrogen deficits. Pesquisa Agropecuária Brasileira, Brasília, v. 42, n. 4, p. 599-601, abr. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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8. | | GUELFI, A.; CHABREGAS, S. M.; FALCO, M. C.; FALCAO, P. R. K.; NESHICH, G.; AZEVEDO, R. A. Molecular dynamics analysis of glutathione transferases sugarcane mutants and their interactions with atrazine. In: X-MEETING; INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu. [Proceedings...]. [S.l.]: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 94. X-meeting 2005. Presented posters.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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9. | | AZEVEDO, R. A. de; ARAÚJO, L.; COELHO, S. G.; FARIA FILHOI, D. E. de; DUARTE, E. R.; GERASEEV, L. C. Desempenho de bezerros alimentados com silagem de leite de transição. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 5, p. 545-552, maio 2013. Título em inglês: Performance of calves fed silage transition milk.Biblioteca(s): Embrapa Unidades Centrais. |
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10. | | MARTINOTTO, F.; MARTINOTTO, C.; COELHO, M. de F. B.; AZEVEDO, R. A B. e; ALBUQUERQUE, M. C. de F. e. Sobrevivência e crescimento inicial de espécies arbóreas nativas do Cerrado em consórcio com mandioca. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 1, p. 22-29, jan. 2012. Título em inglês: Survival and initial growth of tree species native to the Cerrado intercropped with cassava.Biblioteca(s): Embrapa Unidades Centrais. |
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13. | | FURINI, P. M.; SANTOS, G. B.; AZEVEDO, R. A.; RUFINO, S. R. de A.; CARVALHO, B. C. de; MACHADO, F. S.; CAMPOS, M. M.; COELHO, S. G. Avaliação do desenvolvimento da glândula mamária de bezerras mestiças leiteiras até os 90 dias de idade por meio de ultrassonografia. In: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 4 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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14. | | JOLOMBA, J. R.; MARCONDES, M. I.; VELOSO, C. M.; OSS, D. B.; AZEVEDO, R. A.; COELHO, S. G.; CAMPOS, M. M. Breed effect on energy requirements of crossbred (Holstein x Gyr) or purebred Holstein calves. In: SIMPÓSIO NACIONAL DE BOVINOCULTURA LEITEIRA - SIMLEITE, 5.; SIMPÓSIO INTERNACIONAL DE BOVINOCULTURA LEITEIRA, 3., 2015, Viçosa, MG. Anais... Viçosa: UFV, 2015. p. 381-383.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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15. | | GHELFI, A.; GAZIOLA, S. A.; CIA, M. C.; CHABREGAS, S. M.; FALCO, M. C.; KUSER-FALCÃO, P. R.; AZEVEDO, R. A. Cloning, expression, molecular modelling and docking analysis of glutathione transferase from Saccharum officinarum. Annals of Applied Biology, Cambridge, v. 159, n. 2, p. 267-280, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agricultura Digital. |
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16. | | GONÇALVES, F. V.; MEDICI, L. O.; FONSECA, M. P. S. da; GAZIOLA, S. A.; LIMA, G. R.; MACEDO, D. C.; AZEVEDO, R. A. Characterization of the development of cowpea cultivars and of the quantity and quality of proteins in their grains. Pesquisa Agropecuária Brasileira, v. 55, e00837, 2020. Título em português: Caracterização do desenvolvimento de cultivares de feijão-caupi e do conteúdo e da qualidade proteica dos seus grãos.Biblioteca(s): Embrapa Unidades Centrais. |
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17. | | AZEVEDO, R. A.; MACHADO, F. S.; CAMPOS, M. M.; FURINI, P. M.; RUFINO, S. R.; PEREIRA, L. G. R.; TOMICH, T. R.; COELHO, S. G. The effects of increasing amounts of milk replacer powder added to whole milk on feed intake and performance in dairy heifers. Journal of Dairy Science, v. 99, n. 10, p. 8018-8027, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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20. | | AZEVEDO, R. A. de; RUFINO, L. M. de A.; SANTOS, A. C. R. dos; SILVA, L. P. da; BONFÁ, H. C.; DUARTE, E. R.; GERASEEV, L. C. Desempenho de cordeiros alimentados com inclusão de torta de macaúba na dieta. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 11, p. 1663-1668, nov. 2012. Título em inglês: Performance of lambs fed with macauba pie inclusion in the dietBiblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 43 | |
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